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Nanopatterned collagen tubes for vascular tissue engineering
Author(s) -
Zorlutuna P.,
Hasirci N.,
Hasirci V.
Publication year - 2008
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.99
Subject(s) - scanning electron microscope , immunostaining , tissue engineering , materials science , biomedical engineering , fluorescence microscope , electron microscope , nanotechnology , chemistry , fluorescence , pathology , immunohistochemistry , composite material , medicine , physics , optics , quantum mechanics
Nanopatterned (330 nm wide channels) type I collagen films were prepared by solvent casting on poly(dimethyl siloxane) (PDMS) templates. These films were rolled into tubular constructs and crosslinked. Tubular constructs were incubated under cell culture conditions for 28 days and examined by stereomicroscopy and scanning electron microscopy (SEM) for the integrity of the structure. The nanopatterned films were also seeded with human vascular smooth muscle cells (VSMCs) and examined after immunostaining with fluorescence microscopy and SEM to assess the cell phenotype and alignment on the nanopatterns on the films. Copyright © 2008 John Wiley & Sons, Ltd.