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Collagen nanofibres are a biomimetic substrate for the serum‐free osteogenic differentiation of human adipose stem cells
Author(s) -
Sefcik Lauren S.,
Neal Rebekah A.,
Kaszuba Stephanie N.,
Parker Anna M.,
Katz Adam J.,
Ogle Roy C.,
Botchwey Edward A.
Publication year - 2008
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.85
Subject(s) - osteonectin , osteopontin , osteocalcin , extracellular matrix , alkaline phosphatase , chemistry , mesenchymal stem cell , microbiology and biotechnology , stem cell , adipose tissue , cellular differentiation , in vitro , matrix (chemical analysis) , immunology , biochemistry , biology , enzyme , gene , chromatography
Electrospinning has recently gained widespread attention as a process capable of producing nanoscale fibres that mimic native extracellular matrix. In this study, we compared the osteogenic differentiation behaviour of human adipose stem cells (ASCs) on a 3D nanofibre matrix of type I rat tail collagen (RTC) and a 2D RTC collagen‐coated substrate, using a novel serum‐free osteogenic medium. The serum‐free medium significantly enhanced the numbers of proliferating cells in culture, compared to ASCs in traditional basal medium containing 10% animal serum, highlighting a potential clinical role for in vitro stem cell expansion. Osteogenic differentiation behaviour was assessed at days 7, 14 and 21 using quantitative real‐time RT–PCR analysis of the osteogenic genes collagen I ( Coll I ), alkaline phosphatase ( ALP ), osteopontin ( OP ), osteonectin ( ON ), osteocalcin ( OC ) and core‐binding factor‐α ( cbfa1 ). All genes were upregulated (>one‐fold) in ASCs cultured on nanofibre scaffolds over 2D collagen coatings by day 21. Synthesis of mineralized extracellular matrix on the scaffolds was assessed on day 21 with Alizarin red staining. These studies demonstrate that 3D nanoscale morphology plays a critical role in regulating cell fate processes and in vitro osteogenic differentiation of ASCs under serum‐free conditions. Copyright © 2008 John Wiley & Sons, Ltd.