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Monosize polycationic nanoparticles as non‐viral vectors for gene transfer to HeLa cells
Author(s) -
Güven Güldem Utkan,
Laçin Nelisa Türkoǧlu,
Pişkin Erhan
Publication year - 2008
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.78
Subject(s) - hela , gene transfer , nanoparticle , chemistry , virology , gene , microbiology and biotechnology , computational biology , biophysics , nanotechnology , biology , materials science , cell , biochemistry
Monosized cationic nanoparticles were produced by emulsifier‐free emulsion polymerization of styrene, poly(ethylene glycol) methacrylate and N‐[3‐(dimethyl‐amino) propyl] methacrylamide, conducted in the presence of a cationic initiator, 2,2′‐azobis (2‐methylpropionamidine) dihydrochloride, using different amounts of ingredients and at different conditions. The structure of the terpolymers was confirmed by 1 H‐NMR and FTIR spectroscopy. The nanoparticles, with an average size of 71.3 nm [polydispersity index (PDI), 1.110] and a Zeta potential of 65.6 mV obtained by a zeta sizer, were used in the transfection studies. HeLa cells were transfected in in vitro cell cultures with these non‐viral nanoparticle vectors with a green fluorescent protein (GFP)‐expressing plasmid DNA. The transfer of the cationic nanoparticles into the cells and GFP expressions with the conjugates of the nanoparticles and the GFP‐expressing plasmid were followed by both light and fluorescent microscopy. The GFP expression efficiency was unexpectedly high (up to 90%). Copyright © 2008 John Wiley & Sons, Ltd.