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Bone regeneration induced by adenoviral vectors carrying til‐1/Cbfa1 genes implanted with biodegradable porous materials in animal models of osteonecrosis of the femoral head
Author(s) -
Sakai Shinsuke,
Tamura Miki,
Mishima Hajime,
Kojima Hiroko,
Uemura Toshimasa
Publication year - 2008
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.72
Subject(s) - regeneration (biology) , femoral head , biomedical engineering , gene delivery , genetic enhancement , in vivo , microbiology and biotechnology , tissue engineering , scaffold , chemistry , anatomy , gene , medicine , biology , biochemistry
Abstract A new approach for bone regeneration is needed for idiopathic osteonecrosis of the femoral head (ION). Core binding factor α1 (Cbfa1) was reported in 1997 as the most important transcription factor for osteoblastic differentiation. The transgenics of transcription factors affecting bone formation might be useful tools for the bone regeneration. The purpose of this study was to investigate the effects of the implantation of adenoviral vectors carrying Cbfa1 genes implanted with biodegradable porous materials on bone formation in an animal model of ION. Robust and rapid bone regeneration in large bone defects was achieved with the implantation of adenoviral vectors carrying Cbfa1 genes. These results suggest that the Cbfa1 genes induce a rapid osteoblastic differentiation and the biodegradable scaffold successfully functioned as a delivery vehicle for the Cbfa1 gene, as they induced osteogenic repair in vivo , even in necrotic bone. Copyright © 2008 John Wiley & Sons, Ltd.