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Bone regenerative properties of injectable PGLA–CaP composite with TGF‐β1 in a rat augmentation model
Author(s) -
Plachokova Adelina,
Link Dennis,
van den Dolder Juliette,
van den Beucken Jeroen,
Jansen John
Publication year - 2007
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.59
Subject(s) - plga , composite number , chemistry , bone healing , biomedical engineering , h&e stain , rat model , transforming growth factor , dentistry , materials science , immunohistochemistry , medicine , surgery , composite material , in vitro , biochemistry
Abstract The aim of this study was to examine the bone augmentation properties of an injectable composite consisting of PLGA microspheres/CaP cement (20/80), and the additional effect of loading PLGA microspheres with TGF‐β1 (200 ng). For this purpose, PLGA/CaP composites (control) and PLGA/CaP composites loaded with TGF‐β1 (test group) were injected on top of the skulls of 24 Wistar rats. Each rat received 2 materials from the same experimental group, and in total 48 implants were placed (n = 8). After 2, 4, and 8 weeks the results were evaluated histologically and histomorphometrically. The contact length between the implants and newly formed bone increased in time, and was significantly higher for the TGF‐β1‐loaded composites after 2 weeks. Also, bone formation was significantly higher for the TGF‐β1‐loaded composites (18.5% ± 3) compared to controls (7.21% ± 5) after 8 weeks of implantation. Immunohistochemical staining demonstrated massive inflammatory infiltrates in both groups, particularly at 2 weeks, which decreased substantially at 4 and 8 weeks. In conclusion, injectable PLGA/CaP composites stimulated bone augmentation in a rat model. The addition of TGF‐β1 to the composite significantly increased bone contact at 2 weeks and enhanced new bone formation at 8 weeks. Copyright © 2008 John Wiley & Sons, Ltd.