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Control of the timing and dosage of IGF‐I delivery from encapsulated cells
Author(s) -
Patel Roshni S.,
Chang Amy,
Lysaght Michael J.,
Morgan Jeffrey R.
Publication year - 2013
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.546
Subject(s) - doxycycline , growth factor , chemistry , wound healing , tetracycline , microbiology and biotechnology , cell culture , recombinant dna , tissue engineering , pharmacology , biomedical engineering , biology , biochemistry , immunology , gene , antibiotics , medicine , receptor , genetics
Abstract We report here on the development and characterization of a cell‐based system for the regulated delivery of bioactive insulin‐like growth factor I (IGF‐I). A stable mammalian cell line, CHO‐K1 Tet‐IGFI, was genetically modified to have tetracycline‐induced transcription of the human IGF‐I gene. Cells were activated to express IGF‐I in the presence of doxycycline (DOX), a tetracycline derivative, while expression was inactivated in the absence of DOX. Temporal, or on‐off, release of IGF‐I from cells encapsulated within Ca 2+ ‐alginate hydrogels was demonstrated in a pilot study over the course of 10 days in culture. Released growth factor was bioactive, exhibiting a proliferative effect comparable to recombinant purified IGF‐I protein. The dosage levels and temporal control of IGF‐I release from encapsulated cells meet the requirements of orthopedic wound repair, making this approach an attractive means for the controlled synthesis and delivery of growth factors in situ for wound healing. Copyright © 2012 John Wiley & Sons, Ltd.