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Micro‐ and nanostructured hydroxyapatite–collagen microcarriers for bone tissue‐engineering applications
Author(s) -
Perez R. A.,
Altankov G.,
JorgeHerrero E.,
Ginebra M. P.
Publication year - 2013
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.530
Subject(s) - microcarrier , alkaline phosphatase , chemistry , chemical engineering , osteoblast , apatite , bioceramic , tissue engineering , materials science , biomedical engineering , nanotechnology , mineralogy , biochemistry , cell , in vitro , medicine , engineering , enzyme
Novel hydroxyapatite (HA)–collagen microcarriers (MCs) with different micro/nanostructures were developed for bone tissue‐engineering applications. The MCs were fabricated via calcium phosphate cement (CPC) emulsion in oil. Collagen incorporation in the liquid phase of the CPC resulted in higher MC sphericity. The MCs consisted of a porous network of entangled hydroxyapatite crystals, formed as a result of the CPC setting reaction. The addition of collagen to the MCs, even in an amount as small as 0.8 wt%, resulted in an improved interaction with osteoblast‐like Saos‐2 cells. The micro/nanostructure and the surface texture of the MCs were further tailored by modifying the initial particle size of the CPC. A synergistic effect between the presence of collagen and the nanosized HA crystals was found, resulting in significantly enhanced alkaline phosphatase activity on the collagen‐containing nanosized HA MCs. Copyright © 2012 John Wiley & Sons, Ltd.