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Modulation of human mesenchymal stem cell function in a three‐dimensional matrix promotes attenuation of adverse remodelling after myocardial infarction
Author(s) -
Simpson David L.,
Dudley Samuel C.
Publication year - 2013
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.511
Subject(s) - mesenchymal stem cell , myocardial infarction , ventricle , cardiology , stem cell , in vivo , chemistry , biomedical engineering , cardiac function curve , microbiology and biotechnology , cell , matrix (chemical analysis) , medicine , heart failure , biology , biochemistry , chromatography
The application of tissue engineering (TE) practices for cell delivery offers a unique approach to cellular cardiomyoplasty. We hypothesized that human mesenchymal stem cells (hMSCs) applied to the heart in a collagen matrix would outperform the same cells grown in a monolayer and directly injected for cardiac cell replacement after myocardial infarction in a rat model. When hMSC patches were transplanted to infarcted hearts, several measures for left ventricle (LV) remodelling and function were improved, including fractional area change, wall thickness, –dP/dt and LV end‐diastolic pressure. Neovessel formation throughout the LV infarct wall after hMSC patch treatment increased by 37% when compared to direct injection of hMSCs. This observation was correlated with increased secretion of angiogenic factors, with accompanying evidence that these factors enhanced vessel formation (30% increase) and endothelial cell growth (48% increase) in vitro . These observations may explain the in vivo observations of increased vessel formation and improved cardiac function with patch‐mediated cell delivery. Although culture of hMSC in collagen patches enhanced angiogenic responses, there was no effect on cell potency or viability. Therefore, hMSCs delivered as a cardiac patch showed benefits above those derived from monolayers and directly injected. hMSCs cultured and delivered within TE constructs may represent a good option to maximize the effects of cellular cardiomyoplasty. Copyright © 2011 John Wiley & Sons, Ltd.