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Aggregation promotes cell viability, proliferation, and differentiation in an in vitro model of injection cell therapy
Author(s) -
Bayoussef Zahia,
Dixon James E.,
Stolnik Snjezana,
Shakesheff Kevin M.
Publication year - 2012
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.482
Subject(s) - cell , c2c12 , viability assay , cell therapy , microbiology and biotechnology , cell growth , in vitro , chemistry , matrix (chemical analysis) , biophysics , biology , biochemistry , myogenesis , chromatography
Many cell therapy approaches aim to deliver high‐density single‐cell suspensions to diseased or injured sites in the body. Long term clinical success will in part be dependent on the cells that remain viable and that assume correct functionality post‐administration. The research presented in this paper focuses on the potential of cell aggregate delivery to generate a more supportive environment for cells than single cell suspensions. An in vitro model of injection delivery of C2C12 myoblast cells showed a significant difference in cell function and phenotype between adhesive collagen and non‐adhesive alginate, indicating that in vitro assays based on this approach can discriminate between cell‐cell/cell‐matrix interactions and could be valuable when assessing cell therapy systems. Contrary to single cells, aggregates maintain viability, cellular activity, and phenotype beyond that of single cells, even in non‐adhesive matrices, enabling delivery of higher cell densities with enhanced proliferative and differentiation capacity. Copyright © 2011 John Wiley & Sons, Ltd.