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On‐line monitoring of oxygen as a non‐destructive method to quantify cells in engineered 3D tissue constructs
Author(s) -
Santoro R.,
Krause C.,
Martin I.,
Wendt D.
Publication year - 2012
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.473
Subject(s) - bioreactor , biomedical engineering , tissue engineering , cartilage , cell culture , biochemical engineering , biological system , chemistry , biology , engineering , anatomy , genetics , organic chemistry
Regulatory guidelines have established the importance of introducing quantitative quality controls during the production and/or at the time of release of cellular grafts for clinical applications. In this study we aimed to determine whether on‐line measurements of oxygen can be used as a non‐destructive method to estimate the number of chondrocytes within an engineered cartilage graft. Human chondrocytes were seeded and cultured in a perfusion bioreactor, and oxygen levels in the culture medium were continuously monitored at the inlet and outlet of the bioreactor chamber throughout the culture period. We found that the drop in oxygen across the perfused construct was linearly correlated with the number of cells per construct ( R 2 = 0.82, p < 0.0001). The method was valid for a wide range of cell numbers, including cell densities currently used in the manufacture of cartilage grafts for clinical applications. Given that few or no non‐destructive assays that quantitatively characterize an engineered construct currently exist, this non‐invasive method could represent a relevant instrument in regulatory compliant manufacturing of engineered grafts meeting specific quality criteria. Copyright © 2012 John Wiley & Sons, Ltd.