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Promising new potential for mesenchymal stem cells derived from human umbilical cord Wharton's jelly: sweat gland cell‐like differentiative capacity
Author(s) -
Xu Yongan,
Huang Sha,
Ma Kui,
Fu Xiaobing,
Han Weidong,
Sheng Zhiyong
Publication year - 2012
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.468
Subject(s) - mesenchymal stem cell , wharton's jelly , sweat gland , cd90 , stem cell , cd44 , biology , cd34 , microbiology and biotechnology , immunology , cell , sweat , paleontology , genetics
Mesenchymal stem cells derived from Wharton's jelly of the human umbilical cord (hUC‐MSCs) possess various advantageous properties, similar to bone marrow‐derived mesenchymal stem cells (BM‐MSCs), including self‐renewal, extended proliferation potential and multilineage differentiation potential. In this study, we hoped to determine whether hUC‐MSCs could be induced to differentiate into sweat gland cell‐like cells, that would be potential in sweat glands restoration after injury. In this study, the results of flow cytometry analysis revealed that hUC‐MSCs showed the typical antigen profile of MSCs and were positive for CD29, CD44, CD90, CD105 and Oct‐4; they were negative for the antigens of CD34, CEA and CK14. Remarkably, hUC‐MSCs maintained proper proliferation and differentiation ability. After culture in sweat gland cell‐conditioned medium (induction group 1) for 3 weeks, hUC‐MSCs possessed sweat gland cell‐like morphology and expressed markers of sweat gland cells (CEA, CK14 and CK19) more efficiently than those of induction group 2. In reverse‐transcription PCR and western blotting analysis, it was further confirmed that induced hUC‐MSCs (group 1) also expressed a higher level of sweat gland developmental genes ( EDA and EDAR ) than group 2. These results together provided evidence that hUC‐MSCs could possess a new emerging potential to differentiate into sweat gland cell‐like cells with a higher efficacy under our new induction system. Thus, hUC‐MSCs could be considered a new strategy for sweat glands restoration after skin injury as well as improvement of cutaneous regeneration. Copyright © 2011 John Wiley & Sons, Ltd.