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Effect of native and NH 3 plasma‐functionalized polymeric membranes on the gene expression profiles of primary hepatocytes
Author(s) -
Pavlica Sanja,
Schmitmeier Stephanie,
Gloeckner Pia,
Piscioneri Antonella,
Peinemann Frank,
Krohn Knut,
SiegmundSchulz Michaela,
Laera Stefania,
Favia Pietro,
De Bartolo Loredana,
Bader Augustinus
Publication year - 2012
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.453
Subject(s) - gene expression , membrane , bioartificial liver device , microbiology and biotechnology , hepatocyte , gene , cell , microarray analysis techniques , chemistry , in vitro , microarray , cell culture , biology , biochemistry , genetics
Little is known about how cells respond to different biomaterials at the molecular level. Biomaterials could stimulate specific cellular responses at the molecular level, such as activation of signalling pathways that control gene activity involved in the maintenance, growth and functional regeneration of liver tissue in vitro. This aspect is an important step in liver tissue engineering. Currently, there are no data available concerning the modulation of cellular genomic response by using synthetic membranes in a bioartificial system. For the first time we investigated gene expression profiles of primary hepatocytes cultured on different substrates: collagen sandwich, native and NH 3 plasma‐grafted PEEK–WC–PU membranes. Gene expression in cell suspension prepared after cell isolation was used as a control. Generally, microarray data revealed that the expression of the majority of genes remained unchanged compared to the control. Among 31 000 genes, 52 were significantly changed: 20 were upregulated and 32 downregulated. There were similar changes in gene expression of hepatocytes cultured in the membranes and collagen sandwich. However, some genes involved in the cell proliferation and functional metabolic pathways are more expressed in cells cultured on the membranes and especially on the functionalized ones. Both membranes sustained liver functions at the molecular level, demonstrating their suitability for the reconstruction of liver and as a toxicogenomic tool to predict the liver response to novel drugs. Copyright © 2011 John Wiley & Sons, Ltd.