Premium
Flat and tubular membrane systems for the reconstruction of hippocampal neuronal network
Author(s) -
Morelli Sabrina,
Piscioneri Antonella,
Salerno Simona,
Rende Maria,
Campana Carla,
Tasselli Franco,
di Vito Anna,
Giusi Giuseppina,
Caaco Marcello,
Drioli Enrico,
Bartolo Loredana De
Publication year - 2012
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.434
Subject(s) - membrane , hippocampal formation , neurite , microbiology and biotechnology , in vitro , chemistry , adhesion , biomedical engineering , biophysics , materials science , biology , neuroscience , biochemistry , medicine , organic chemistry
Abstract The selection of appropriate biomaterials that promote cellular adhesion and growth is particularly important for the in vitro reconstruction of neuronal network. This study focused on the development of new polymeric membranes in flat and tubular (hollow‐fibre) configurations as novel biomaterials for neuronal outgrowth. Two membrane systems constituted by modified polyetheretherketone (PEEK‐WC) and polyacrylonitrile (PAN) membranes were developed and used for the culture of hamster hippocampal neurons. We demonstrated that all investigated membranes supported the adhesion and growth of hippocampal neurons enhancing neuronal differentiation and neurite alignment. The differences in cell behaviours between cells cultured on flat and hollow‐fibre (HF) membranes were highlighted by the quantitative analysis of neuronal marker fluorescence intensity, morphometric analysis, RT–PCR analysis and also by metabolic activity measurements. In particular, the PAN HF membranes showed ideal growth culture conditions, guaranteeing adequate levels of metabolic features. Primary hippocampal cells cultured on PAN HF membranes were able to recreate in vitro a 3D neural tissue‐like structure that, mimicking the hippocampal tissue, could be used as a tool for the study of natural and pathological neurobiological events. Copyright © 2011 John Wiley & Sons, Ltd.