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Engineered tendon with decellularized xenotendon slices and bone marrow stromal cells: an in vivo animal study
Author(s) -
Omae Hiromichi,
Sun Yu Long,
An KaiNan,
Amadio Peter C.,
Zhao Chunfeng
Publication year - 2012
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.423
Subject(s) - decellularization , tendon , stromal cell , in vivo , histology , biomedical engineering , tissue engineering , chemistry , bone marrow , anatomy , pathology , medicine , biology , microbiology and biotechnology
The purpose of this study was to investigate an engineered composite of multilayer acellular tendon slices seeded with bone marrow stromal cells (BMSCs) as a possible solution for tendon reconstruction. BMSCs were harvested from 15 rabbits and infraspinatus tendons were harvested from 17 dogs. The decellularized tendons were sectioned in longitudinal slices with a thickness of 50 µm. The BMSCs were seeded on the slices and then the slices were bundled into one composite. The composite was implanted into a rabbit patellar tendon defect. Tendon slices without BMSCs were implanted into the contralateral patellar tendon as a control. The composites were evaluated by histology and qRT–PCR. The viability of BMSCs was assessed using a fluorescent marker. Histology showed viable cells between the collagen fibres on the cell‐seeded side. Analysis by qRT–PCR showed higher tenomodulin, collagen type III, MMP3 and MMP13 expressions and lower collagen type I expression in the cell‐seeded composite than in the tendon slices without BMSCs. We conclude that BMSCs can survive in a multilayer composite, express a tendon phenotype and enhance the metabolism of tendon in vivo . This in vivo study suggests a potential utility of this composite in tendon reconstruction. Copyright © 2011 John Wiley & Sons, Ltd.