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Human fetal bone cells in delivery systems for bone engineering
Author(s) -
Tenorio Diene M. H.,
Scaletta Corinne,
Jaccoud Sandra,
HirtBurri Nathalie,
Pioletti Dominique P.,
Jaques Bertrand,
Applegate Lee Ann
Publication year - 2011
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.381
Subject(s) - biocompatibility , tissue engineering , von kossa stain , hyaluronic acid , biomedical engineering , chemistry , bone cell , fetus , microbiology and biotechnology , medicine , anatomy , biology , pregnancy , alkaline phosphatase , biochemistry , genetics , organic chemistry , enzyme
Abstract The aim of this study was to culture human fetal bone cells (dedicated cell banks of fetal bone derived from 14 week gestation femurs) within both hyaluronic acid gel and collagen foam, to compare the biocompatibility of both matrices as potential delivery systems for bone engineering and particularly for oral application. Fetal bone cell banks were prepared from one organ donation and cells were cultured for up to 4 weeks within hyaluronic acid (Mesolis ® ) and collagen foams (TissueFleece ® ). Cell survival and differentiation were assessed by cell proliferation assays and histology of frozen sections stained with Giemsa, von Kossa and ALP at 1, 2 and 4 weeks of culture. Within both materials, fetal bone cells could proliferate in three‐dimensional structure at ∼70% capacity compared to monolayer culture. In addition, these cells were positive for ALP and von Kossa staining, indicating cellular differentiation and matrix production. Collagen foam provides a better structure for fetal bone cell delivery if cavity filling is necessary and hydrogels would permit an injectable technique for difficult to treat areas. In all, there was high biocompatibility, cellular differentiation and matrix deposition seen in both matrices by fetal bone cells, allowing for easy cell delivery for bone stimulation in vivo . Copyright © 2011 John Wiley & Sons, Ltd.