Premium
Effects of different sera on adipose tissue‐derived mesenchymal stromal cells
Author(s) -
Tunaitis Virginijus,
Borutinskaitė Veronika,
Navakauskienė Rūta,
Treigytė Gražina,
Ungurytė Aušra,
Aldonytė Rūta,
Magnusson KarlEric,
Pivoriūnas Augustas
Publication year - 2011
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.374
Subject(s) - mesenchymal stem cell , adipose tissue , adipogenesis , stromal cell , biology , fetal bovine serum , in vitro , andrology , microbiology and biotechnology , immunology , medicine , cancer research , endocrinology , biochemistry
Current cell therapy protocols require considerable numbers of mesenchymal stromal cells (MSCs), which can be obtained only by in vitro expansion. The most important issue is a choice of optimal growth supplements for cell culture. Ideally, these should be of known composition, free of animal components and allow production of large homogenic populations of MSCs in a considerably short period of time. Since this standard has not been achieved to date, we aimed to assess the molecular responses of MSCs to different growth supplements commonly in use. MSCs were isolated from breast or abdominal adipose tissue and plated into DMEM supplemented with one of four different sera: fetal calf serum (FCS), pretested fetal calf serum (FCS‐Sp), human allogeneic serum (HS) or artificial serum substitute (AS). MSCs cultivated with different serum supplements demonstrated distinct morphologies, high adipogenic and osteogenic differentiation potential and expressed characteristic antigens. Using real‐time PCR, we found a large increase in PPAR γ and Msx2 gene expression in both lines of proliferating MSCs cultivated with AS. We found that MSCs cultivated in the presence of different sera had similar global proteomic expression patterns, but comparisons of identified proteins revealed most differences in the MSCs cultivated with AS. Our results indicate that MSCs cultivated in the presence of FCS and HS display similar growth, differentiation, immunophenotypic and proteomic properties, while AS induces more profound changes in the physiology of MSCs, suggesting that further fundamental studies should be done before its introduction into clinical practice. Copyright © 2010 John Wiley & Sons, Ltd.