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The effect of ex vivo dynamic loading on the osteogenic differentiation of genetically engineered mesenchymal stem cell model
Author(s) -
KimelmanBleich Nadav,
Seliktar Dror,
Kallai Ilan,
Helm Gregory A.,
Gazit Zulma,
Gazit Dan,
Pelled Gadi
Publication year - 2011
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.324
Subject(s) - mesenchymal stem cell , bone morphogenetic protein 2 , microbiology and biotechnology , self healing hydrogels , ex vivo , chemistry , alkaline phosphatase , tissue engineering , in vivo , stem cell , biomedical engineering , in vitro , biochemistry , biology , enzyme , medicine , organic chemistry
Mechanical loading has been described as a highly important stimulus for improvements in the quality and strength of bone. It has also been shown that mechanical stimuli can induce the differentiation of mesenchymal stem cells (MSCs) along the osteogenic lineage. We have previously demonstrated the potent osteogenic effect of MSCs engineered to overexpress the BMP2 gene. In this study we investigated the effect of mechanical loading on BMP2‐expressing MSC‐like cells, using a special bioreactor designed to apply dynamic forces on cell‐seeded hydrogels. Cell viability, alkaline phosphatase (ALP) activity, BMP2 secretion and mineralized substance formation in the hydrogels were quantified. We found that cell metabolism increased as high as 6.8‐fold, ALP activity by 12.5‐fold, BMP2 secretion by 182‐fold and mineralized tissue formation by 1.72‐fold in hydrogels containing MSC‐like cells expressing BMP2, which were cultured in the presence of mechanical loading. We have shown that ex vivo mechanical loading had an additive effect on BMP2‐induced osteogenesis in genetically engineered MSC‐like cells. These data could be valuable for bone tissue‐engineering strategies of the future. Copyright © 2010 John Wiley & Sons, Ltd.