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In vivo effectiveness of hybrid membranes with osteogenic growth peptide for bone regeneration
Author(s) -
OshiroJunior João Augusto,
Barros Rafaela Moreno,
Silva Camila Garcia,
Souza Caroline Cordeiro,
Scardueli Cássio Rocha,
Marcantonio Camila Chiérici,
Silva Saches Paulo Ricardo,
Mendes Larissa,
Cilli Eduardo Maffud,
Marcantonio Rosemary Adriana Chierici,
Chiavacci Leila Aparecida
Publication year - 2021
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.3226
Subject(s) - membrane , small angle x ray scattering , differential scanning calorimetry , in vivo , biomedical engineering , tissue engineering , biophysics , chemistry , materials science , regeneration (biology) , biochemistry , microbiology and biotechnology , scattering , biology , medicine , thermodynamics , physics , optics
Guided bone regeneration (GBR) technique helps to restore bone tissue through cellular selectivity principle. Currently no osteoinductive membrane exists on the market. Osteogenic growth peptide (OGP) acts as a hematopoietic stimulator. This association could improve the quality of bone formation, benefiting more than 2.2 million patients annually. The objective of this work was to develop membranes from ureasil‐polyether materials containing OGP. The membranes were characterized by differential scanning calorimetry (DSC) and small angle X‐ray scattering (SAXS). OGP was synthesized by the solid phase method. Sterilization results using gamma radiation at 24 kGy did not change the structure of the material, as confirmed by DSC. The SAXS technique revealed the structural homogeneity of the matrix. OGP was incorporated in 66.25 × 10 −10 mol and release results showed that the ureasil‐PPO400/PEO500 and ureasil‐PPO400/PEO1900 membranes released 7% and 21%, respectively, after 48 h. In vivo results demonstrated that the amount and quality of bone tissue formed in the bone defects in the presence of ureasil‐polyether membranes with OGP were similar to commercial collagen material with BMP. The results allow us to conclude that membranes with OGP have characteristics that make them potential candidates for the GBR.