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In situ functionalization of wet‐spun fibre meshes for bone tissue engineering
Author(s) -
Leonor Isabel B.,
Rodrigues Márcia T.,
Gomes Manuela E.,
Reis Rui L.
Publication year - 2011
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.294
Subject(s) - polycaprolactone , biomedical engineering , surface modification , tissue engineering , in situ , materials science , chemistry , chemical engineering , composite material , engineering , polymer , organic chemistry
Bone tissue engineering success strongly depends on our ability to develop new materials combining osteoconductive, osteoinductive and osteogenic properties. Recent studies suggest that biomaterials incorporating silanol (SiOH) groups promote and maintain osteogenesis. The purpose of the present research work was to provide evidence that using wet‐spinning technologies and a calcium silicate solution as a coagulation bath, it was possible to develop an in situ functionalization methodology to obtain 3D wet‐spun fibre meshes with SiOH groups, through a simple, economic and reliable process. SPCL (blend of starch with polycaprolactone) fibre meshes were produced by wet‐spinning, using a calcium silicate solution as a non‐solvent and functionalized in situ with SiOH groups. In vitro tests, using goat bone marrow stromal cells (GBMSCs), showed that SPCL–Si scaffolds sustained cell viability and proliferation. Furthermore, high ALP activity and matrix production indicated that SiOH groups improve cellular functionality towards the osteoblastic phenotype. Using this methodology, and assembling several wet‐spun fibre meshes, 3D meshes can be developed, aiming at designing osteoconductive/osteoinductive 3D structures capable of stimulating bone ingrowth in vivo . Copyright © 2010 John Wiley & Sons, Ltd.