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Electrical pulse stimulation of skeletal myoblasts cell cultures with simulated action potentials
Author(s) -
Villanueva Paula,
Pereira Sheila,
Olmo Alberto,
Pérez Pablo,
Yuste Yaiza,
Yúfera Alberto,
Portilla Fernando
Publication year - 2019
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2869
Subject(s) - skeletal muscle , stimulation , myocyte , pulse (music) , biomedical engineering , in vitro , electrophysiology , chemistry , materials science , biophysics , biology , voltage , microbiology and biotechnology , anatomy , neuroscience , physics , medicine , biochemistry , quantum mechanics
Electrical pulse stimulation has an important effect on skeletal muscle development and maturation. However, the methodology for controlling these stimulation parameters to develop in vitro functional skeletal muscle tissues remains to be established. In this work, we have studied the effect of simulated action potentials on the growth and differentiation of skeletal myoblast cell cultures. A circuit simulating action potentials of 0.15 and 0.3 V/mm, at a frequency of 1 Hz and with a 4‐ms pulse width, is proposed. Results show an important improvement of the growth rate and differentiation of myoblasts at a voltage of 0.15 V/mm. Parameters such as electrodes geometry or type of signals must be considered in the development of in vitro skeletal muscle.