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Precision cell delivery in biphasic polymer systems enhances growth of keratinocytes in culture and promotes their attachment on acellular dermal matrices
Author(s) -
Agarwal Rishima,
Liu Guanyong,
Tam Nicky W.,
Gratzer Paul F.,
Frampton John P.
Publication year - 2019
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2845
Subject(s) - keratinocyte , polyethylene glycol , polymer , chemistry , biophysics , dextran , tissue engineering , biomedical engineering , materials science , cell culture , peg ratio , substrate (aquarium) , microbiology and biotechnology , in vitro , biology , chromatography , biochemistry , composite material , engineering , ecology , genetics , finance , economics
Current approaches for precision deposition of cells are not optimized for moist environments or for substrates with complex surface topographic features, for example, the surface of dermal matrices and other biomaterials. To overcome these challenges, an approach is presented that utilizes cell confinement in phase‐separating polymer solutions of polyethylene glycol and dextran to precisely deliver keratinocytes in well‐defined colonies. Using this approach, keratinocyte colonies are produced with superior viability, proliferative capacity, and barrier formation compared with the same number of cells dispersedly seeded across substrate surfaces. It is further demonstrated that keratinocytes delivered in colonies to the surface of acellular dermal matrices form an intact epidermal basal layer more rapidly and more completely than cells delivered by conventional dispersed seeding. These findings demonstrate that delivery of keratinocytes in phase‐separating polymer solutions holds potential for enhancing growth of keratinocytes in culture and production of functional skin equivalents.