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Hyaline cartilage next generation implants from adipose‐tissue–derived mesenchymal stem cells: Comparative study on 3D‐printed polycaprolactone scaffold patterns
Author(s) -
Theodoridis Konstantinos,
Aggelidou Eleni,
Vavilis Theofanis,
Manthou Maria Eleni,
Tsimponis Antonios,
Demiri Efterpi C.,
Boukla Anna,
Salpistis Christos,
Bakopoulou Athina,
Mihailidis Athanassios,
Kritis Aristeidis
Publication year - 2019
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2798
Subject(s) - scaffold , chondrogenesis , biomedical engineering , polycaprolactone , mesenchymal stem cell , hyaline cartilage , cartilage , materials science , tissue engineering , adipose tissue , chemistry , anatomy , microbiology and biotechnology , biology , composite material , engineering , pathology , articular cartilage , medicine , biochemistry , alternative medicine , osteoarthritis , polymer
We used additive manufacturing to fabricate 3D‐printed polycaprolactone scaffolds of different geometry topologies and porosities. We present a comparative analysis of hyaline cartilage development from adipose‐tissue–derived mesenchymal stem cells (ADMSCs) on three different, newly designed scaffold geometry patterns. The first scaffold design (MESO) was based on a rectilinear layer pattern. For the second pattern (RO45), we employed a 45° rotational layer loop. The design for the third scaffold (3DHC) was a three‐dimensional honeycomb‐like pattern with a hexagonal cellular distribution and small square shapes. We examined cell proliferation, colonization, and differentiation, in relation to the scaffold's structure, as well as to the mechanical properties of the final constructs. We gave emphasis on the scaffolds, both microarchitecture and macroarchitecture, for optimal and enhanced chondrogenic differentiation, as an important parameter, not well studied in the literature. Among the three patterns tested, RO45 was the most favourable for chondrogenic differentiation, whereas 3DHC better supported cell proliferation and scaffold penetration, exhibiting also the highest rate of increase onto the mechanical properties of the final construct. We conclude that by choosing the optimal scaffold architecture, the resulting properties of our cartilaginous constructs can better approximate those of the physiological cartilage.