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Cyclosporine‐impregnated allograft bone sterilized with low‐temperature plasma
Author(s) -
Lu Haibo,
Pei Guoxian,
Zhao Peiran,
Liang Shuangwu,
Jin Dan,
Jiang Shan
Publication year - 2010
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.279
Subject(s) - immunosuppression , bone marrow , bone healing , immunohistochemistry , chemistry , pathology , andrology , stromal cell , medicine , urology , surgery
Deep‐freezing, freeze‐drying and gamma (γ)‐irradiation have deleterious effects on bone healing and mechanical properties of allograft bones. We tried preparing bone allografts using cyclosporine plus low‐temperature‐plasma sterilization. To explore the feasibility of this method of preparation, segmental defects in the right radii of rabbits were repaired with cyclosporine‐impregnated allograft bones (CABs) sterilized with low‐temperature‐plasma (in the study group) and deep‐frozen/freeze‐dried irradiated allograft bones (D/FIABs) (in the control group). X‐ray and quantitative histological analysis, peripheral blood T lymphocyte subset analysis and CD 25 molecule immunohistochemistry stain, the four‐point bending test and safety evaluations were respectively conducted to compare bone‐healing, immunosuppression, mechanical properties and safety between the two groups. X‐ray scores were higher in the study group than those in the control ( p = 0.032). There were significant differences in new bone areas at most repairs in both groups ( p < 0.05). There were no significant differences in the percentages of CD 4 + T, CD 8 + T, ratios of CD 4 + T:CD 8 + T or serum concentrations of GPT/Cr in both groups ( p > 0.05). At 16 weeks postoperatively, the density of CD 25 molecules in the control group was higher than that in the study group. The ultimate loading in the study group was significantly higher than that in the control ( p = 0.048). Bone marrow stromal cells (BMSCs) grew thickly around and on the surface of a cyclosporine‐impregnated allograft. Livers and kidneys in the study and control groups remained histologically normal at 7 days postoperatively. These results indicate that the CAB might be a better material than the D/FIAB in terms of bone healing, preservation of mechanical properties and immunosuppression without severe side‐effects. Copyright © 2010 John Wiley & Sons, Ltd.