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Gap closure of different shape wounds: In vitro and in vivo experimental models in the presence of engineered protein adhesive hydrogel
Author(s) -
Thanikachalam Thiruselvi,
Selvaraj Thirupathi Kumara Raja,
Ayyappan Manikandan,
Arumugam Gnanamani
Publication year - 2019
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2779
Subject(s) - in vivo , wound healing , biomedical engineering , biophysics , in vitro , rectangle , materials science , chemistry , fibroblast , wound closure , mathematics , geometry , biology , engineering , biochemistry , immunology , microbiology and biotechnology
The present study emphasizes the role of engineered protein (gallic acid engineered gelatin [GEG]) on the closure of wound gaps of different shapes assessed under in vitro (fibroblast cell line) and in vivo (rat) experimental models. Circular, triangle, rectangle, and square are the shapes selected for the study. Intending engineered protein (GEG) augments the cell migration in rectangle and triangle shapes and reduces the gap space significantly compared with circular and square shapes. Similar observations were made with in vivo model study, and it was observed that the wound closure starts along the wound edges. In circular and square shapes, the cell movement follow a purse‐string mechanism/the mixed pattern. Thus, the present study suggested that for faster wound healing, the cell migration along the wound edge may be found beneficial, and the external healing agent in the form of engineered protein hydrogel accelerate the healing accordingly.