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Fabrication and evaluation of carbonate apatite‐coated calcium carbonate bone substitutes for bone tissue engineering
Author(s) -
FujiokaKobayashi Masako,
Tsuru Kanji,
Nagai Hirokazu,
Fujisawa Kenji,
Kudoh Takaharu,
Ohe Go,
Ishikawa Kunio,
Miyamoto Youji
Publication year - 2018
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2742
Subject(s) - chemistry , resorption , apatite , calcium , calcium carbonate , dissolution , artificial bone , bone tissue , biomedical engineering , mineralogy , medicine , organic chemistry
Carbonate apatite‐coated calcium carbonate (CO 3 Ap/CaCO 3 ) was fabricated through a dissolution–precipitation reaction using CaCO 3 granules as a precursor to accelerate bone replacement based on superior osteoconductivity of the CO 3 Ap shell, along with Ca 2+ release from the CaCO 3 core and quicker resorption of the CaCO 3 core. In the present study, CaCO 3 , 10% CO 3 Ap/CaCO 3 , 30% CO 3 Ap/CaCO 3 , and CO 3 Ap granules were fabricated and examined histologically to evaluate their potential as bone substitutes. Larger contents of CaCO 3 in the granules resulted in higher Ca 2+ release and promoted cell proliferation of murine preosteoblasts at 6 days compared with CO 3 Ap. Interestingly, in a rabbit femur defect model, 10% CO 3 Ap/CaCO 3 induced significantly higher new bone formation and higher material resorption compared with CO 3 Ap at 8 weeks. Nevertheless, CO 3 Ap showed a superior osteoconductive potential compared with 10% CO 3 Ap/CaCO 3 at 8 weeks. All tested granules were most likely resorbed by cell mediation including multinucleated giant cell functions. Therefore, we conclude that CO 3 Ap/CaCO 3 has a positive potential for bone tissue engineering based on well‐controlled calcium release, bone formation, and material resorption.