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Activated platelet‐rich plasma improves cartilage regeneration using adipose stem cells encapsulated in a 3D alginate scaffold
Author(s) -
Beigi MohammadHossein,
Atefi Atefeh,
Ghanaei HamidReza,
Labbaf Sheyda,
Ejeian Fatemeh,
NasrEsfahani MohammadHossein
Publication year - 2018
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2663
Subject(s) - chondrogenesis , cartilage , aggrecan , chemistry , platelet rich plasma , mesenchymal stem cell , adipose tissue , fibrocartilage , hyaline cartilage , regeneration (biology) , extracellular matrix , microbiology and biotechnology , glycosaminoglycan , stem cell , matrix (chemical analysis) , scaffold , anatomy , biomedical engineering , pathology , osteoarthritis , biology , platelet , immunology , medicine , biochemistry , articular cartilage , alternative medicine , chromatography
In the current study, the effect of superimposing platelet‐rich plasma (PRP) on different culture mediums in a three‐dimensional alginate scaffold encapsulated with adipose‐derived mesenchymal stem cells for cartilage tissue repair is reported. The three‐dimensional alginate scaffolds with co‐administration of PRP and/or chondrogenic supplements had a significant effect on the differentiation of adipose mesenchymal stem cells into mature cartilage, as assessed by an evaluation of the expression of cartilage‐related markers of Sox9, collagen II, aggrecan and collagen, and glycosaminoglycan assays. For in vivo studies, following induction of osteochondral lesion in a rabbit model, a high degree of tissue regeneration in the alginate plus cell group (treated with PRP plus chondrogenic medium) compared with other groups of cell‐free alginate and untreated groups (control) were observed. After 8 weeks, in the alginate plus cell group, functional chondrocytes were observed, which produced immature matrix, and by 16 weeks, the matrix and hyaline‐like cartilage became completely homogeneous and integrated with the natural surrounding cartilage in the defect site. Similar effect was also observed in the subchondral bone. The cell‐free scaffolds formed fibrocartilage tissue, and the untreated group did not form a continuous cartilage over the defect by 16 weeks.