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Human progenitor‐derived endothelial cells vs. venous endothelial cells for vascular tissue engineering: an in vitro study
Author(s) -
Thebaud Noélie B.,
Bareille Reine,
Remy Murielle,
Bourget Chantal,
Daculsi Richard,
Bordenave Laurence
Publication year - 2010
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.261
Subject(s) - tissue engineering , progenitor cell , umbilical vein , microbiology and biotechnology , endothelial stem cell , in vitro , endothelial progenitor cell , cell culture , vascular tissue , chemistry , biomedical engineering , immunology , biology , stem cell , medicine , biochemistry , botany , genetics
The isolation of endothelial progenitor cells from human peripheral blood generates a great hope in vascular tissue engineering because of particular benefit when compared with mature endothelial cells. We explored the capability of progenitor‐derived endothelial cells (PDECs) to line fibrin and collagen scaffolds in comparison with human saphenous and umbilical cord vein endothelial cells (HSVECs and HUVECs): (a) in a static situation, allowing definition of the optimal cell culture conditions with different media and cell‐seeding densities to check cell behaviour; (b) under shear stress conditions (flow chambers or tubular vascular constructs), allowing investigation of cell response and mRNA expression on both substrates by oligonucleotide microarray analysis and quantitative real‐time PCR. Well characterized PDECs: (a) could not be expanded adequately with the usual mature ECs culture media; (b) were able to colonize and grow on fibrin glue; (c) exhibited higher resistance to oxidative stress than HSVECs and HUVECs; (d) withstood physiological shear stress when lining both substrates in flow chambers, and their gene expression was regulated; (e) colonized a collagen‐impregnated vascular prosthesis and were able to sense mechanical forces. Our results provide an improved qualification of PDECs for vascular tissue engineering. Copyright © 2010 John Wiley & Sons, Ltd.