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Chitosan‐based hydrogels do not induce angiogenesis
Author(s) -
Ahmadi Raheleh,
Burns Alan J.,
de Bruijn Joost D.
Publication year - 2010
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.247
Subject(s) - angiogenesis , chitosan , mesenchymal stem cell , self healing hydrogels , chemistry , chorioallantoic membrane , biomedical engineering , tissue engineering , neovascularization , microbiology and biotechnology , biochemistry , cancer research , medicine , biology , polymer chemistry
The aim of this study was to assess the angiogenic potential of chitosan‐glycerol phosphate (GP)‐hydroxyethyl cellulose (HEC) binder for injectable bone tissue engineering applications. The angiogenic response of chitosan‐GP‐HEC combined with and without human bone marrow‐derived mesenchymal stem cells (hMSCs) was examined using the chick chorioallantoic membrane (CAM) assay. Chitosan‐GP‐HEC gel did not show any angiogenic potential, whereas the presence of hMSCs gave rise to an enhanced angiogenic response when placed on the CAM for 3 days. Quantitatively, significantly more blood vessel formation was observed for the stem cell‐containing group as compared to all other groups ( p < 0.05 ), except for the b‐FGF‐positive control. The results indicate that the chitosan‐GP‐HEC binder does not contribute to enhanced angiogenesis and that the presence of hMSCs enhances angiogenesis. Copyright © 2009 John Wiley & Sons, Ltd.