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Bio‐inspired solute enables preservation of human oocytes using minimum volume vitrification
Author(s) -
Choi Jung Kyu,
El Assal Rami,
Ng Nicholas,
Ginsburg Elizabeth,
Maas Richard L.,
Anchan Raymond M.,
Demirci Utkan
Publication year - 2018
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2439
Subject(s) - vitrification , cryoprotectant , cryopreservation , dimethyl sulfoxide , andrology , fertility preservation , biology , chemistry , embryo , microbiology and biotechnology , fertility , medicine , population , environmental health , organic chemistry
The ability to cryopreserve human oocytes has significant potential for fertility preservation. Current cryopreservation methods still suffer from the use of conventional cryoprotectants, such as dimethyl sulphoxide (DMSO), causing loss of viability and function. Such injuries result from the toxicity and high concentration of cryoprotectants, as well as mechanical damage of cells due to ice crystal formation during the cooling and rewarming processes. Here we report the preservation of human oocytes following vitrification using an innovative bio‐inspired cryoprotectant integrated with a minimum volume vitrification approach. The results demonstrate that the recovered human oocytes maintained viability following vitrification and rewarming. Moreover, when this approach was used to vitrify mouse oocytes, the recovered oocytes preserved their viability and function following vitrification and rewarming. This bio‐inspired approach substitutes DMSO, a well‐known toxic cryoprotectant, with ectoine, a non‐toxic naturally occurring solute. The bio‐inspired vitrification approach has the potential to improve fertility preservation for women undergoing cancer treatment and endangered mammal species.

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