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Potential effect of mechano growth factor E‐domain peptide on axonal guidance growth in primary cultured cortical neurons of rats
Author(s) -
Liu Meili,
Niu Xufeng,
Zhou Gang,
Jia Zhengtai,
Li Ping,
Fan Yubo
Publication year - 2018
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2364
Subject(s) - netrin , neuroprotection , western blot , nerve growth factor , immunohistochemistry , chemistry , microbiology and biotechnology , in vivo , growth cone , receptor , pharmacology , biology , axon , biochemistry , axon guidance , immunology , gene
Abstract Establishing appropriate synaptic connections and plasticity is a critical need in neuronal regeneration and development. Mechano growth factor (MGF) and its C‐terminal E‐domain peptide with 24 amino acids, MGF‐Ct24E, are potential neuroprotective agents. Our preliminary study indicates that Netrin‐1 can guide axonal growth and its expression is sensitive to MGF, but how MGF regulates the expression of Netrin‐1 and its receptor DCC is still unclear. Here, we investigate the effect of MGF‐Ct24E on the expression of Netrin‐1 and DCC in primary cultured cortical neurons in vitro and the adult rat brain in vivo . MTT assay shows that MGF‐Ct24E can significantly protect primary cortical neurons against nerve injury. There is a significant increase in axonal elongation after MGF‐Ct24E treatment at concentrations of 0.5 and 1.0 μg/ml. Real‐time polymerase chain reaction assay indicates that MGF‐Ct24E can effectively promote the expression of Netrin‐1 and DCC in primary cultured cortical neurons. To identify the certain mechanism of MGF‐Ct24E on neuronal guidance and growth, adult rats are subjected to intramuscular injection of MGF‐Ct24E after traumatic brain injury. Rats injected with MGF‐Ct24E start eating and drinking within 14 days, indicating that MGF‐Ct24E can promote rehabilitation. HE staining and immunohistochemistry assays of brain section slices reveal that MGF‐Ct24E treatment can significantly inhibit the haemorrhage of traumatic brain injury and promote expression of Netrin‐1. Further investigation of protein expression by Western blot assay shows that MGF‐Ct24E promotes expression of Netrin‐1 and DCC after nerve injury. MGF‐Ct24E can effectively improve axonal guidance through upregulation of Netrin‐1/DCC signalling in neuronal regeneration. Copyright © 2016 John Wiley & Sons, Ltd.