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Engineered neural tissue with Schwann cell differentiated human dental pulp stem cells: potential for peripheral nerve repair?
Author(s) -
Sanen Kathleen,
Martens Wendy,
Georgiou Melanie,
Ameloot Marcel,
Lambrichts Ivo,
Phillips James
Publication year - 2017
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2249
Subject(s) - dental pulp stem cells , neurite , nerve guidance conduit , regeneration (biology) , sciatic nerve , in vivo , tissue engineering , microbiology and biotechnology , peripheral nerve , neural tissue engineering , stem cell , peripheral nerve injury , schwann cell , biomedical engineering , in vitro , anatomy , pathology , chemistry , medicine , biology , biochemistry
Despite the spontaneous regenerative capacity of the peripheral nervous system, large gap peripheral nerve injuries (PNIs) require bridging strategies. The limitations and suboptimal results obtained with autografts or hollow nerve conduits in the clinic urge the need for alternative treatments. Recently, we have described promising neuroregenerative capacities of Schwann cells derived from differentiated human dental pulp stem cells (d‐hDPSCs) in vitro . Here, we extended the in vitro assays to show the pro‐angiogenic effects of d‐hDPSCs, such as enhanced endothelial cell proliferation, migration and differentiation. In addition, for the first time we evaluated the performance of d‐hDPSCs in an in vivo rat model of PNI. Eight weeks after transplantation of NeuraWrap™ conduits filled with engineered neural tissue (EngNT) containing aligned d‐hDPSCs in 15‐mm rat sciatic nerve defects, immunohistochemistry and ultrastructural analysis revealed ingrowing neurites, myelinated nerve fibres and blood vessels along the construct. Although further research is required to optimize the delivery of this EngNT, our findings suggest that d‐hDPSCs are able to exert a positive effect in the regeneration of nerve tissue in vivo . Copyright © 2017 John Wiley & Sons, Ltd.