Premium
Towards reconstruction of epithelialized cartilages from autologous adipose tissue‐derived stem cells
Author(s) -
New Sophie E.P.,
Ibrahim Amel,
Guasti Leonardo,
Zucchelli Eleonora,
Birchall Martin,
Bulstrode Neil W.,
Seifalian Alexander M.,
Ferretti Patrizia
Publication year - 2017
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2211
Subject(s) - stem cell , mesenchymal stem cell , tissue engineering , adipose tissue , microbiology and biotechnology , chondrogenesis , biomedical engineering , cartilage , chemistry , anatomy , biology , medicine , biochemistry
Deformities of the upper airways, including those of the nose and throat, are typically corrected by reconstructive surgery. The use of autologous somatic stem cells for repair of defects could improve quality and outcomes of such operations. The present study explored the ability of paediatric adipose‐derived stem cells (pADSCs), a readily available source of autologous stem cells, to generate a cartilage construct with a functional epithelium. Paediatric ADSCs seeded on the biodegradable nanocomposite polymer, polyhedral oligomeric silsesquioxane poly(ϵ‐caprolactone‐urea) urethane (POSS‐PCL), proliferated and differentiated towards mesenchymal lineages. The ADSCs infiltrated three‐dimensional POSS‐PCL nanoscaffold and chondroid matrix was observed throughout chondrogenically induced samples. In ovo chorioallantoic membrane‐grafted ADSC‐nanoscaffold composites were enwrapped by host vessels indicating good compatibility in an in vivo system. Furthermore, pADSCs could be induced to transdifferentiate towards barrier‐forming epithelial‐like cells. By combining differentiation protocols, it was possible to generate epithelial cell lined chondrogenic micromasses from the same pADSC line. This proof‐of‐concept study appears to be the first to demonstrate that individual pADSC lines can differentiate towards two different germ lines and be successfully co‐cultured. This has important implications for bioengineering of paediatric airways and further confirms the plastic nature of ADSCs. Copyright © 2016 John Wiley & Sons, Ltd.