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Characterization of human adipose tissue‐derived stem cells with enhanced angiogenic and adipogenic properties
Author(s) -
Lauvrud Anne Therese,
Kelk Peyman,
Wiberg Mikael,
Kingham Paul J.
Publication year - 2017
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2147
Subject(s) - cd146 , adipose tissue , mesenchymal stem cell , stromal vascular fraction , stem cell , adipogenesis , population , stromal cell , tissue engineering , angiogenesis , microbiology and biotechnology , stem cell transplantation for articular cartilage repair , chemistry , endothelial stem cell , biology , immunology , in vitro , adult stem cell , cancer research , medicine , endocrinology , cd34 , biomedical engineering , biochemistry , environmental health
Abstract Autologous fat grafting is a popular method for soft tissue reconstructions but graft survival remains highly unpredictable. Supplementation of the graft with the stromal vascular fraction (SVF) or cultured adipose tissue‐derived stem cells (ASCs) can enhance graft viability. In this study we have examined the phenotypic properties of a selected population of cells isolated from ASCs, with a view to determining their suitability for transplantation into grafts. ASCs were isolated from the SVF of human abdominal fat ( n = 8 female patients) and CD146 + cells were selected using immunomagnetic beads. The angiogenic and adipogenic properties of the positively selected cells were compared with the negative fraction. CD146 + cells expressed the immunophenotypic characteristics of pericytes. With prolonged in vitro expansion, CD146 − cells exhibited increased population doubling times and morphological signs of senescence, whereas CD146 + cells did not. CD146 + cells expressed higher levels of the angiogenic molecules VEGF‐A, angiopoietin‐1 and FGF‐1. Conditioned medium taken from CD146 + cells significantly increased formation of in vitro endothelial cell tube networks, whereas CD146 − cells did not. CD146 + cells could be differentiated into adipocytes in greater numbers than CD146 − cells. Consistent with this, differentiated CD146 + cells expressed higher levels of the adipocyte markers adiponectin and leptin. These results suggest that CD146 + cells selected from a heterogeneous mix of ASCs have more favourable angiogenic and adipogenic properties, which might provide significant benefits for reconstructive and tissue‐engineering applications. Copyright © 2016 John Wiley & Sons, Ltd.