Premium
Collagen type I–PLGA film as an efficient substratum for corneal endothelial cells regeneration
Author(s) -
Kim Eun Young,
Tripathy Nirmalya,
Cho Sun Ah,
Lee Dongwon,
Khang Gilson
Publication year - 2017
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2145
Subject(s) - plga , biocompatibility , regeneration (biology) , biomedical engineering , chemistry , cornea , biophysics , tissue engineering , corneal transplantation , regenerative medicine , in vitro , microbiology and biotechnology , materials science , cell , ophthalmology , biology , biochemistry , medicine , organic chemistry
Surface modulations of desired biological construct design for regenerative medical therapy is considered to be highly crucial for cell growth and the subsequent regeneration of biologically competent tissues. In this study, we fabricated stable, transparent, collagen type‐I‐coated PLGA films (Col I–PLGA) as a potential substratum for the regeneration of corneal endothelial cells. Morphological and structural properties were analysed by FE–SEM, AFM, FTIR, contact angle, etc., and in vitro biocompatibility of the Col I–PLGA films was further tested in primary rabbit corneal endothelial cells (rCEnCs) as models. Compared with bare PLGA films, the Col I–PLGA films displayed the requisite surface roughness, with higher Ra (nm) values, transparency, good hydrophilicity, stability and water uptake. Next, cultured rCEnCs on Col I–PLGA films showed the characteristic polygonal shape of rCEnCs with enhanced initial attachment, proliferation and expression of mRNAs. Collectively, these results indicate that Col I–PLGA can be employed as a suitable alternative for high‐quality corneal tissue expansion and transplantation. Copyright © 2016 John Wiley & Sons, Ltd.