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Scaffold–cell bone engineering in a validated preclinical animal model: precursors vs differentiated cell source
Author(s) -
Berner A.,
Henkel J.,
Woodruff M. A.,
Saifzadeh S.,
Kirby G.,
Zaiss S.,
Gohlke J.,
Reichert J. C.,
Nerlich M.,
Schuetz M. A.,
Hutmacher D. W.
Publication year - 2017
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2104
Subject(s) - scaffold , mesenchymal stem cell , in vivo , progenitor cell , bone marrow , regeneration (biology) , tissue engineering , biomedical engineering , skeleton (computer programming) , chemistry , anatomy , stem cell , pathology , microbiology and biotechnology , biology , medicine
The properties of osteoblasts (OBs) isolated from the axial skeleton (tOBs) differ from OBs of the orofacial skeleton (mOBs) due to the different embryological origins of the bones. The aim of the study was to assess and compare the regenerative potential of allogenic bone marrow‐derived mesenchymal progenitor cells with allogenic tOBs and allogenic mOBs in combination with a mPCL–TCP scaffold in critical‐sized segmental bone defects in sheep tibiae. After 6 months, the tibiae were explanted and underwent biomechanical testing, micro‐computed tomography (microCT) and histological and immunohistochemical analyses. Allogenic MPCs demonstrated a trend towards a better outcome in biomechanical testing and the mean values of newly formed bone. Biomechanical, microCT and histological analysis showed no significant differences in the bone regeneration potential of tOBs and mOBs in our in vitro study, as well as in the bone regeneration potential of different cell types in vivo . Copyright © 2015 John Wiley & Sons, Ltd.