Premium
PCL–HA microscaffolds for in vitro modular bone tissue engineering
Author(s) -
Totaro Alessandra,
Salerno Aurelio,
Imparato Giorgia,
Domingo Concepción,
Urciuolo Francesco,
Netti Paolo Antonio
Publication year - 2017
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2084
Subject(s) - polycaprolactone , biomedical engineering , mesenchymal stem cell , chemistry , tissue engineering , bone tissue , in vitro , nanocomposite , materials science , microbiology and biotechnology , nanotechnology , biochemistry , polymer , biology , engineering , organic chemistry
The evolution of microscaffolds and bone‐bioactive surfaces is a pivotal point in modular bone tissue engineering. In this study, the design and fabrication of porous polycaprolactone (PCL) microscaffolds functionalized with hydroxyapatite (HA) nanoparticles by means of a bio‐safe and versatile thermally‐induced phase separation process is reported. The ability of the as‐prepared nanocomposite microscaffolds to support the adhesion, growth and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in standard and osteogenic media and using dynamic seeding/culture conditions was investigated. The obtained results demonstrated that the PCL–HA nanocomposite microparticles had an enhanced interaction with hMSCs and induced their osteogenic differentiation, even without the exogenous addition of osteogenic factors. In particular, calcium deposition, alizarin red assay, histological analysis, osteogenic gene expression and collagen I secretion were assessed. The results of these tests demonstrated the formation of bone microtissue precursors after 28 days of dynamic culture. These findings suggest that PCL–HA nanocomposite microparticles represent an excellent platform for in vitro modular bone tissue engineering. Copyright © 2015 John Wiley & Sons, Ltd.