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A nanofibrous cell‐seeded hydrogel promotes integration in a cartilage gap model
Author(s) -
Maher S. A.,
Mauck R. L.,
Rackwitz L.,
Tuan R. S.
Publication year - 2010
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.205
Subject(s) - cartilage , chondrocyte , chemistry , seeding , biomedical engineering , transforming growth factor , matrix (chemical analysis) , chondrogenesis , articular cartilage , cell growth , cell , biophysics , materials science , microbiology and biotechnology , osteoarthritis , anatomy , pathology , biology , biochemistry , medicine , alternative medicine , agronomy , chromatography
The presence of a defect in mature articular cartilage can lead to degenerative changes of the joint. This is in part caused by the inability of cartilage to regenerate tissue that is capable of spanning a fissure or crack. In this study, we hypothesized that introduction of a biodegradable cell‐seeded nanofibrous hydrogel, Puramatrix ™ , into a cartilage gap would facilitate the generation of a mechanically stable interface. The effects of chondrocyte incorporation within the hydrogel and supplementation with transforming growth factor‐β3 (TGFβ3), a known regulator of cell growth and differentiation, on cartilage integration were examined mechanically and histologically as a function of cell density and incubation time. When supplemented with TGFβ3, the cell‐seeded hydrogel exhibited abundant matrix generation within the hydrogel and a corresponding increase in maximum push‐out stress as compared to all other groups. Furthermore, initial cell seeding density affected interfacial strength in a time‐dependent manner. This study suggests that a cell‐seeded TGFβ3‐supplemented hydrogel can encourage integration between two opposing pieces of articular cartilage. Copyright © 2009 John Wiley & Sons, Ltd.