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Treatment by Therapeutic Magnetic Resonance (TMR™) increases fibroblastic activity and keratinocyte differentiation in an in vitro model of 3D artificial skin
Author(s) -
Ferroni Letizia,
Bellin Gloria,
Emer Valeria,
Rizzuto Rosario,
Isola Maurizio,
Gardin Chiara,
Zavan Barbara
Publication year - 2017
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2031
Subject(s) - dermis , fibroblast , extracellular matrix , in vitro , dermal fibroblast , epidermis (zoology) , microbiology and biotechnology , chemistry , skin equivalent , wound healing , matrix (chemical analysis) , mtt assay , biomedical engineering , keratinocyte , anatomy , biology , immunology , medicine , biochemistry , chromatography
This study investigated the effect of extremely low‐frequency pulsed electromagnetic fields (PEMFs) on skin wound healing in an in vitro dermal‐like tissue. In this study, fibroblast and endothelial cells were utilized for the in vitro reconstruction of dermal‐like tissues treated for various times up to 21 days with PEMFs. The effects of PEMFs on cell proliferation (MTT test), cell ageing ( β ‐galactosidase test, ROS production), gene expression, the quality of the extracellular matrix and the amount of fibroblast growth factors were analysed. The high quality of the dermis products in the presence of PEMFs at the end of the study was confirmed through the high degree of organization of keratinocytes, which were subsequently seeded on the aforementioned in vitro reconstructed dermis. The cells organized themselves in well‐defined multi‐layers and were better organized compared with the epidermis present on the dermis that was obtained without PEMF treatment. Copyright © 2015 John Wiley & Sons, Ltd.