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TGF β 3 secretion by three‐dimensional cultures of human dental apical papilla mesenchymal stem cells
Author(s) -
Somoza Rodrigo A.,
Acevedo Cristian A.,
Albornoz Fernando,
LuzCrawford Patricia,
Carrión Flavio,
Young Manuel E.,
WeinsteinOppenheimer Caroline
Publication year - 2017
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.2004
Subject(s) - mesenchymal stem cell , secretion , microbiology and biotechnology , regeneration (biology) , tissue engineering , dental papilla , regenerative medicine , stem cell , extracellular matrix , chemistry , cell , biology , biomedical engineering , pathology , pulp (tooth) , medicine , biochemistry , odontoblast
Mesenchymal stem cells (MSCs) can be isolated from dental tissues, such as pulp and periodontal ligament; the dental apical papilla (DAP) is a less‐studied MSC source. These dental‐derived MSCs are of great interest because of their potential as an accessible source for cell‐based therapies and tissue‐engineering (TE) approaches. Much of the interest regarding MSCs relies on the trophic‐mediated repair and regenerative effects observed when they are implanted. TGF β 3 is a key growth factor involved in tissue regeneration and scarless tissue repair. We hypothesized that human DAP‐derived MSCs (hSCAPs) can produce and secrete TGF β 3 in response to micro‐environmental cues. For this, we encapsulated hSCAPs in different types of matrix and evaluated TGF β 3 secretion. We found that dynamic changes of cell–matrix interactions and mechanical stress that cells sense during the transition from a monolayer culture (two‐dimensional, 2D) towards a three‐dimensional (3D) culture condition, rather than the different chemical composition of the scaffolds, may trigger the TGF β 3 secretion, while monolayer cultures showed almost 10‐fold less secretion of TGF β 3. The study of these interactions is provided as a cornerstone in designing future strategies in TE and cell therapy that are more efficient and effective for repair/regeneration of damaged tissues. Copyright © 2015 John Wiley & Sons, Ltd.