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A silk‐based encapsulation platform for pancreatic islet transplantation improves islet function in vivo
Author(s) -
Hamilton Diana C.,
Shih Hank H.,
Schubert Richard A.,
Michie Sara A.,
Staats Paul N.,
Kaplan David L.,
Fontaine Magali J.
Publication year - 2017
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1990
Subject(s) - islet , mesenchymal stem cell , in vivo , transplantation , cell encapsulation , silk , streptozotocin , pancreatic islets , biocompatibility , chemistry , andrology , medicine , endocrinology , cell , diabetes mellitus , pathology , biology , biochemistry , materials science , microbiology and biotechnology , organic chemistry , composite material
The success of pancreatic islet (PI) transplantation is challenged by PI functional damage during the peritransplantation period. A silk‐based encapsulation platform including mesenchymal stromal cells (MSCs) was evaluated for islet cell delivery in vivo . Islet equivalents (IEQs) were transplanted into the epididymal fat pads of mice with streptozotocin‐induced diabetes. Three PI combinations were tested: (A) co‐encapsulated in silk with MSCs; (b) encapsulated in silk alone; or (c) pelleted. Blood glucose levels were monitored and intraperitoneal glucose tolerance test (IPGTT) was performed upon return to euglycaemia. Grafts were removed for histology and cytokine content analysis. Mice with PI grafts in silk showed a prompt return to euglycaemia. IPGTT was significantly improved with PI in silk with MSCs, compared to PI in silk alone or pelleted. Both Th 1 and Th 2 cytokines were increased in PI grafts in silk, but Th 1 cytokines were decreased significantly with PI and MSC co‐encapsulation. Histological analysis showed osteogenesis and chondrogenesis in the silk grafts containing MSCs. Future studies will evaluate MSC stability and function in vivo and improve silk biocompatibility for applications in islet transplantation. Copyright © 2015 John Wiley & Sons, Ltd.