Premium
Meniscal repair in vivo using human chondrocyte‐seeded PLGA mesh scaffold pretreated with platelet‐rich plasma
Author(s) -
Kwak Hong Suk,
Nam Jinwoo,
Lee Jihye,
Kim Hee Joong,
Yoo Jeong Joon
Publication year - 2017
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1938
Subject(s) - plga , scaffold , platelet rich plasma , chemistry , in vivo , biomedical engineering , fibrin , chondrocyte , tissue engineering , meniscus , in vitro , platelet , medicine , immunology , biology , biochemistry , physics , microbiology and biotechnology , incidence (geometry) , optics
The objective of this study was to test the hypothesis that platelet‐rich plasma (PRP) pretreatment on a poly‐lactic‐co‐glycolic acid (PLGA) mesh scaffold enhances the healing capacity of the meniscus with human chondrocyte‐seeded scaffolds in vivo, even when the seeded number of cells was reduced from 10 million to one million. A flexible PLGA mesh scaffold was pretreated with PRP using a centrifugal technique. One million human articular chondrocytes were seeded onto the scaffold by dynamic oscillation. After 7 days, scaffolds were placed between human meniscal discs and were implanted subcutaneously in nude mice for 6 weeks ( n = 16/group). Fluorescence microscopy demonstrated uniform attachment of the chondrocytes throughout the scaffolds 24 h following seeding. Cell attachment analysis revealed a significantly increased number of chondrocytes on PRP‐pretreated than non‐treated scaffolds ( p < 0.05). Field emission scanning electron microscopy revealed chondrocytes attached to the PRP‐pretreated scaffolds interconnecting their cellular processes with the fibrin network at 24 h and day 7 of culture. Of the 16 constructs containing PRP‐pretreated scaffolds implanted in mice, six menisci healed completely, nine healed incompletely and one did not heal. Histological results from the 16 control constructs containing non‐treated scaffolds revealed that none had healed completely, four healed incompletely and 12 did not heal. The histological outcome between the groups was significantly different ( p < 0.05). These findings suggest that human articular chondrocytes on PRP‐pretreated PLGA mesh scaffolds demonstrate increased cell attachment and enhance the healing capacity of meniscus with a reduced number of seeding cells in a meniscal repair mouse model. Copyright © 2014 John Wiley & Sons, Ltd.