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Organotypic culture to assess cell adhesion, growth and alignment of different organs on silk fibroin
Author(s) -
Duval JeanLuc,
Dinis Tony,
Vidal Guillaume,
Vigneron Pascale,
Kaplan David L.,
Egles Christophe
Publication year - 2017
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1916
Subject(s) - fibroin , silk , biomaterial , bombyx mori , cell adhesion , adhesion , tissue engineering , biomedical engineering , cell culture , sericin , cell growth , materials science , chemistry , microbiology and biotechnology , nanotechnology , biology , composite material , engineering , biochemistry , genetics , gene
Glass sheets covered with aligned electrospun silk fibroin ( Bombyx mori ) were compared to tissue culture‐treated Thermanox® coverslips, using an organotypic culture method. Different chick embryo organ behaviours were analysed in terms of circularity, cell growth and cell adhesion after being cultivated in contact with these two materials. The circularity (cell layer shape corresponding to the trend of the biomaterials to induce a specific directionality) depends on the organ used when in contact with silk fibroin. This biomaterial induced higher cell adhesion (kidney) or lower cell adhesion (spine) compared to Thermanox. Cell growth, represented by the cell layer area (mm 2 ), was also drastically reduced (gonad) or increased (blood vessel) on the silk fibroin. Organotypic culture is a rapid, cost effective and relatively simple method to evaluate different parameters, allowing prescreening of morphology and cytocompatibility to select the appropriate applications for new biomaterials. In the present study we compared the morphology of different organotypic cultures on orientated silk and Thermanox as growth supports to rapidly evaluate the benefit of a silk‐based biomaterial for tissue engineering. Copyright © 2014 John Wiley & Sons, Ltd.

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