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Anchoring a cytoactive factor in a wound bed promotes healing
Author(s) -
Chattopadhyay Sayani,
Guthrie Kathleen M.,
Teixeira Leandro,
Murphy Christopher J.,
Dubielzig Richard R.,
McAnulty Jonathan F.,
Raines Ronald T.
Publication year - 2016
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1886
Subject(s) - wound healing , conjugate , chemistry , peptide , biomedical engineering , pharmacology , surgery , medicine , biochemistry , mathematics , mathematical analysis
Wound healing is a complex process that requires the intervention of cytoactive factors. The one‐time application of soluble factors to a wound bed does not maintain a steady, sufficient concentration. Here we investigated the benefits of anchoring a factor in a wound bed via a tether to endogenous collagen. We used a collagen‐mimetic peptide (CMP) as a pylon. The CMP binds to damaged but not intact collagen and thus localizes a pendant cytoactive factor in the regions of a wound bed that require intervention. As a model factor, we chose substance P, a peptide of the tachykinin family that promotes wound healing. Using splinted wounds in db / db mice, we found that the one‐time application of a CMP–substance P conjugate enhances wound healing compared to unconjugated substance P and other controls. Specifically, all 16 wounds treated with the conjugate closed more thoroughly and, did so with extensive re‐epithelialization and mitigated inflammatory activity. These data validate a simple and general strategy for re‐engineering wound beds by the integration of beneficial cytoactive factors. Copyright © 2014 John Wiley & Sons, Ltd.