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Breast epithelial cell infiltration in enhanced electrospun silk scaffolds
Author(s) -
MaghdouriWhite Yas,
Elmore Lynne W.,
Bowlin Gary L.,
Dréau Didier
Publication year - 2016
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1778
Subject(s) - electrospinning , silk , biomedical engineering , infiltration (hvac) , materials science , viability assay , chemistry , composite material , cell , polymer , medicine , biochemistry
In the present study, the effects of air‐flow impedance electrospinning and air‐flow rates on silk‐based scaffolds for biological tissues were investigated. First, the properties of scaffolds obtained from 7% and 12% silk concentrations were defined. In addition, cell infiltration and viability of MCF‐10A breast epithelial cells cultured onto these scaffolds were used to determine the biological suitability of these nanostructures. Air‐flow impedance electrospun scaffolds resulted in an overall larger pore size than scaffolds electrospun on a solid mandrel, with the largest pores in 7% silk electrospun with an air pressure of 100 kPa and in 12% silk electrospun with an air pressure of 400 kPa (13.4 ± 0.67 and 26.03 ± 1.19 µm, respectively). After 14 days in culture, the deepest MCF‐10A cell infiltration (36.58 ± 2.28 µm) was observed into 7% silk air‐flow impedance electrospun scaffolds subjected to an air pressure of 100 kPa. In those scaffolds MCF‐10A cell viability was also highest after 14 days in culture. Together, these results strongly support the use of 7% silk‐based scaffolds electrospun with a 100 kPa air‐flow as the most suitable microenvironment for MCF‐10A infiltration and viability. Copyright © 2013 John Wiley & Sons, Ltd.