Premium
Signalling strategies for osteogenic differentiation of human umbilical cord mesenchymal stromal cells for 3D bone tissue engineering
Author(s) -
Wang Limin,
Singh Milind,
Bonewald Lynda F.,
Detamore Michael S.
Publication year - 2009
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.176
Subject(s) - mesenchymal stem cell , runx2 , tissue engineering , chemistry , microbiology and biotechnology , stromal cell , biomedical engineering , cellular differentiation , umbilical cord , wharton's jelly , bone tissue , osteoblast , immunology , biology , in vitro , medicine , biochemistry , cancer research , gene
Human umbilical cord mesenchymal stromal cells (hUCMSCs) have recently shown the capacity to differentiate into multiple cell lineages in all three embryonic germ layers. The osteogenic differentiation of hUCMSCs in monolayer culture has been reported, while the differentiation in three‐dimensional biomaterials has not yet been reported for tissue‐engineering applications. Thus, the aim of this study was to evaluate the feasibility of using hUCMSCs for bone tissue engineering. hUCMSCs were cultured in poly( L ‐lactic acid) (PLLA) scaffolds in osteogenic medium (OM) for 3 weeks, after which the scaffolds were exposed to several different media, including the OM, a mineralization medium (MM) and the MM with either 10 or 100 ng/ml insulin‐like growth factor (IGF)‐1. The osteogenic differentiation was confirmed by the up‐regulation of Runx2 and OCN , calcium quantification and bone histology. Switching from the OM to the MM promoted collagen synthesis and calcium content per cell, while continuing in the OM retained more cells in the constructs and promoted higher osteogenic gene expression. The addition of IGF‐1 into the MM had no effect on cell proliferation, differentiation and matrix synthesis. In conclusion, hUCMSCs show significant potential for bone tissue engineering and culturing in the OM throughout the entire period is beneficial for osteogenic differentiation of these cells. Copyright © 2009 John Wiley & Sons, Ltd.