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Fabrication of transplantable corneal epithelial and oral mucosal epithelial cell sheets using a novel temperature‐responsive closed culture device
Author(s) -
Nakajima Ryota,
Kobayashi Toyoshige,
Kikuchi Tetsutaro,
Kitano Yuriko,
Watanabe Hiroya,
Mizutani Manabu,
Nozaki Takayuki,
Senda Naoko,
Saitoh Kazuo,
Takagi Ryo,
Yamato Masayuki,
Okano Teruo,
Takeda Shizu
Publication year - 2015
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1728
Subject(s) - cell culture , polycarbonate , epithelium , materials science , microbiology and biotechnology , tissue engineering , biomedical engineering , cell , chemistry , biology , composite material , pathology , medicine , biochemistry , genetics
Temperature‐responsive culture surfaces make it possible to harvest transplantable carrier‐free cell sheets. Here, we applied temperature‐responsive polymer for polycarbonate surfaces with previously developed closed culture devices for an automated culture system in order to fabricate transplantable stratified epithelial cell sheets. Histological and immunohistochemical analyses and colony‐forming assays revealed that corneal epithelial and oral mucosal epithelial cell sheets could be harvested with the temperature‐responsive closed culture devices. The results were similar to those obtained using temperature‐responsive culture inserts. These results indicate that the novel temperature‐responsive closed culture device is useful for fabricating transplantable stratified epithelial cell sheets. Copyright © 2013 John Wiley & Sons, Ltd.