Premium
High‐throughput proteomic characterization of plasma rich in growth factors (PRGF–Endoret)‐derived fibrin clot interactome
Author(s) -
Anitua Eduardo,
Prado Roberto,
Azkargorta Mikel,
RodriguezSuárez Eva,
Iloro Ibon,
CasadoVela Juan,
Elortza Felix,
Orive Gorka
Publication year - 2015
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1721
Subject(s) - fibrin , scaffold , regeneration (biology) , wound healing , chemistry , proteomics , scaffold protein , interactome , microbiology and biotechnology , computational biology , biomedical engineering , biology , biochemistry , gene , signal transduction , engineering , immunology
Plasma rich in growth factors (PRGF®‐Endoret®) is an autologous technology that contains a set of proteins specifically addressed to wound healing and tissue regeneration. The scaffold formed by using this technology is a clot mainly composed of fibrin protein, forming a three‐dimensional (3D) macroscopic network. This biomaterial is easily obtained by biotechnological means from blood and can be used in a range of situations to help wound healing and tissue regeneration. Although the main constituent of this clot is the fibrin scaffold, little is known about other proteins interacting in this clot that may act as adjuvants in the healing process. The aim of this study was to characterize the proteins enclosed by PRGF–Endoret scaffold, using a double‐proteomic approach that combines 1D‐SDS–PAGE approach followed by LC–MS/MS, and 2‐DE followed by MALDI–TOF/TOF. The results presented here provide a description of the catalogue of key proteins in close contact with the fibrin scaffold. The obtained lists of proteins were grouped into families and networks according to gene ontology. Taken together, an enrichment of both proteins and protein families specifically involved in tissue regeneration and wound healing has been found. Copyright © 2013 John Wiley & Sons, Ltd.