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Lysophosphatidic acid enhances collagen deposition and matrix thickening in engineered tissue
Author(s) -
Chabaud Stéphane,
Marcoux ThomasLouis,
DeschênesRompré MariePier,
Rousseau Alexandre,
Morissette Amélie,
Bouhout Sara,
Bernard Geneviève,
Bolduc Stéphane
Publication year - 2015
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1711
Subject(s) - lysophosphatidic acid , extracellular matrix , chemistry , microbiology and biotechnology , fibroblast , matrix (chemical analysis) , cell culture , cell , biochemistry , biophysics , in vitro , biology , receptor , genetics , chromatography
The time needed to produce engineered tissue is critical. A self‐assembly approach provided excellent results regarding biological functions and cell differentiation because it closely respected the microenvironment of cells. Nevertheless, the technique was time consuming for producing tissue equivalents with enough extracellular matrix to allow manipulations. Unlike L‐arginine supplementation that only increased accumulation of collagen in cell culture supernatant in our model, addition of lysophosphatidic acid, a natural bioactive lipid, did not modify the amount of accumulated collagen in the cell culture supernatant; however, it enhanced the matrix deposition rate without inducing fibroblast hyperproliferation and tissue fibrosis. Copyright © 2013 John Wiley & Sons, Ltd.