Premium
Multipotent adult progenitor cell‐loaded demineralized bone matrix for bone tissue engineering
Author(s) -
Supronowicz Peter,
Gill Elise,
Trujillo Angelica,
Thula Taili,
Zhukauskas Rasa,
Perry Robert,
Cobb Ronald R.
Publication year - 2016
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1706
Subject(s) - demineralized bone matrix , matrix (chemical analysis) , extracellular matrix , chemistry , tissue engineering , cartilage , biomedical engineering , dbm , materials science , anatomy , medicine , chromatography , biochemistry , amplifier , optoelectronics , cmos
Multipotent adult progenitor cells (MAPCs) from bone marrow have been shown to be capable of forming bone, cartilage and other connective tissues. In addition, MAPCs differentiate into lineages that are different from their germ layers of origin. Previous studies showed the ability of MAPCs to improve cardiac function and control allogenic‐reactive responses associated with acute graft versus host disease. In the current study, we evaluated the ability of MAPCs to produce bone matrix on demineralized bone allograft substrates. Specifically, MAPCs expressed alkaline phosphatase, produced extracellular matrix proteins and deposited calcium‐containing mineral on demineralized bone matrices. Furthermore, the addition of MAPCs on demineralized bone matrix (DBM) scaffolds enhanced osteoinductivity of the carrier in a rat ectopic pouch model. These results demonstrated the potential of MAPCs as a new approach for bone repair in tissue‐engineering applications. Copyright © 2013 John Wiley & Sons, Ltd.