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SIKVAV‐modified highly superporous PHEMA scaffolds with oriented pores for spinal cord injury repair
Author(s) -
Kubinová Šárka,
Horák Daniel,
Hejčl Aleš,
Plichta Zdeněk,
Kotek Jiří,
Proks Vladimír,
Forostyak Serhiy,
Syková Eva
Publication year - 2015
Publication title -
journal of tissue engineering and regenerative medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.835
H-Index - 72
eISSN - 1932-7005
pISSN - 1932-6254
DOI - 10.1002/term.1694
Subject(s) - self healing hydrogels , scaffold , spinal cord injury , spinal cord , biomedical engineering , materials science , tissue engineering , polymer chemistry , medicine , psychiatry
The architecture and mechanical properties of a scaffold for spinal cord injury treatment must provide tissue integration as well as effective axonal regeneration. Previous work has demonstrated the cell‐adhesive and growth‐promoting properties of the SIKVAV (Ser–Ile–Lys–Val–Ala–Val)‐modified highly superporous poly(2‐hydroxethyl methacrylate) (PHEMA) hydrogels. The aim of the current study was to optimize the porosity and mechanical properties of this type of hydrogel in order to develop a suitable scaffold for the repair of spinal cord tissue. Three types of highly superporous PHEMA hydrogels with oriented pores of ~60 µm diameter, porosities of 57–68% and equivalent stiffness characterized by elasticity moduli in the range 3–45 kPa were implanted into a spinal cord hemisection, and their integration into the host tissue, as well as the extent of axonal ingrowth into the scaffold pores, were histologically evaluated. The best tissue response was found with a SIKVAV‐modified PHEMA hydrogel with 68% porosity and a moderate modulus of elasticity (27 kPa in the direction along the pores and 3.6 kPa in the perpendicular direction). When implanted into a spinal cord transection, the hydrogel promoted tissue bridging as well as aligned axonal ingrowth. In conclusion, a prospective oriented scaffold architecture of SIKVAV‐modified PHEMA hydrogels has been developed for spinal cord injury repair; however, to develop an effective treatment for spinal cord injury, multiple therapeutic approaches are needed. Copyright © 2013 John Wiley & Sons, Ltd.